熒光燈與熒光激發(fā)光源的區(qū)別

老師和同學(xué)們經(jīng)常將帶激發(fā)熒光蛋白發(fā)光的燈的稱為熒光燈 - 無(wú)論是夜間潛水觀察海底生物還是在實(shí)驗(yàn)室或野外觀察熒光蛋白 - 稱為"熒光燈"。雖然這沒(méi)有壞處，但它們不是，我們?cè)噲D在技術(shù)上是正確的。

People often refer to lights for bringing out (exciting) fluorescence – whether for night diving or in the lab or field – as ‘fluorescent lights’. While there is no harm in that, they aren’t, and we try to be technically correct.

辦公室的頭頂熒光燈

美國(guó)nightsea 440-460nm藍(lán)光熒光手電筒激發(fā)熒光

"熒光燈"是您在辦公樓中常見(jiàn)的。之所以這樣稱呼它們，是因?yàn)樗鼈兺ㄟ^(guò)熒光發(fā)出白光。管子里裝滿了低壓汞蒸氣。熒光燈的鎮(zhèn)流器輸出電壓激發(fā)汞原子，使它們?cè)谔囟úㄩL(zhǎng)下發(fā)出zui大發(fā)射的電磁輻射。zui強(qiáng)的發(fā)射波長(zhǎng)在254nm處，在紫外線下相當(dāng)?shù)停?65（長(zhǎng)波紫外線），405（紫色），436（藍(lán)色）和546（綠色）nm處有較強(qiáng)的輸出，還有其他波段的光線。如果允許254nm光從燈中逸出，那將是一件壞事，因?yàn)樵摬ㄩL(zhǎng)會(huì)導(dǎo)致皮膚損傷，白內(nèi)障和其他問(wèn)題！該管涂有熒光粉，該熒光粉吸收紫外線并通過(guò)熒光將其轉(zhuǎn)換為寬范圍的波長(zhǎng)。通過(guò)改變熒光粉涂層的化學(xué)成分，制造商可以調(diào)整光譜輸出，使"暖白色"，"冷白色"和其他顏色變化。[附注 - 一些熒光燈管是專(zhuān)門(mén)為發(fā)射254nm光而制造的紫外線燈管，這些紫外線燈是用于對(duì)水和各種表面進(jìn)行shajun和。

‘Fluorescent lights’ are what you commonly find in office buildings. They are called that because they emit their white light by means of fluorescence. The tubes are filled with a low pressure mercury vapor. The electronics that drive the lamp excite the mercury atoms, causing them to emit electromagnetic radiation with maximum emission at specific wavelengths. The strongest emission is at 254nm, fairly far down in the ultraviolet, with additional lines at 365 (longwave UV), 405 (violet), 436 (blue), and 546 (green) nm, plus additional weaker lines. It would be a bad thing if the 254nm light were allowed to escape the lamp since that wavelength can cause skin damage, cataracts, and other problems! The tube is coated with a phosphor that absorbs the UV light and transforms it to a broad range of wavelengths by fluorescence. By varying the chemical composition of the phosphor coating, manufacturers can adjust the spectral output to make ‘warm white’, ‘cool white’, and other color variations. [Side note – some fluorescent tubes are specifically manufactured to emit the 254nm light, and these are used for sterilizing water and various surfaces.]

用于觀察熒光蛋白發(fā)熒光的燈不像辦公室燈那樣通過(guò)熒光起作用 - 它們旨在發(fā)射高強(qiáng)度的單波段光。例如單波段熒光手電筒和便攜式雙波段熒光蛋白激發(fā)光源，美國(guó)nightsea使用高強(qiáng)度發(fā)光二極管（LED）。對(duì)于電子閃光燈組件，美國(guó)nightsea提供定制濾光片，僅允許所需的熒光激發(fā)波長(zhǎng)通過(guò)。這些燈的正確術(shù)語(yǔ)是"熒光激發(fā)光源"或"熒光激發(fā)器"。

The lights for seeing fluorescence do not operate by fluorescence like your office lights – they are designed to cause (excite) other things to fluoresce. For the flashlights and other steady illuminators we use high intensity light-emitting diodes (LEDs). For electronic flash units we provide custom filters that only allow the desired fluorescence excitation wavelengths to pass. A proper terminology for these lights would be ‘fluorescence excitation light’ or ‘fluorescence exciter’, but that’s a mouthful.

上海峰志儀器有限公司現(xiàn)貨供應(yīng)美國(guó)nightsea和美國(guó)路陽(yáng)的熒光手電筒，產(chǎn)品介紹請(qǐng)瀏覽《LUYOR-3280熒光蛋白觀察手電筒》和《美國(guó)nightsea Xite-RB熒光手電筒》。